CYA Medium
(Containing Sucrose 30 g/l, NaNO3 3 g/l, K2HPO4 1.0 g/l, MgSO4 0.5 g/l, KCl 0.5 g/l, FeSO4 0.01 g/l, Yeast Extract 1 g/l and Agar 15 g/l)
CYAS
CYA supplemented with 5% NaCl
MEA Medium
(Containing Maltose Extract 20 g/l, Peptone 1 g/l, Glucose 20 g/l, and Agar 15 g/l)
DRYES: Dichloran Rose Bengal Yeast Extract Sucrose agar (Frisvad, 1983)
Yeast extract 20 g
Sucrose 150 g
Dichloran (0.2% in ethanol) 1.0 ml
Rose bengal (5% soln., w/v) 0.5 ml
Chloramphenicol 0.1 g
Agar 20 g
Water to 1000 ml
Final pH 5.6 (adjusted after medium is autoclaved). This medium detects Penicillium verrucosum and P. viridicatum by production of a purple reverse colour. Can be modified by adding 0.5 g MgSO4.7H2O.
OA: Oatmeal agar (CBS)
Heat 30 g oat flakes in 1 litre water to boiling and simmer gently for 2h. Filter through cloth and fill up to 1 litre. Add 15 g agar to one litre and sterilize by autoclaving at 121°C for 15 min. When using powdered oatmeal, filtering is superfluous. Lupine stems may be placed in slants with oatmeal agar. Also commercially available.
CREA: Creatine Sucrose agar
Creatine(1 H2O) 3 g
Sucrose 30 g
KCl 0.5 g
MgSO4.7H2O 0.5 g
FeSO4.7H2O 0.01 g
K2HPO4.3H2O 1.3 g
Bromocresol purple 0.05 g
Agar 15 g
Distilled water 1000 ml
Final pH 8.0 ± 0.2 (adjust after medium is autoclaved).
NOTE: a modification of CREA (=CRE) with 1.6 g
K3PO4.7H2O can also be used.
DG18: Dichloran 18% Glycerol agar
Peptone 5 g
Glucose 10 g
KH2PO4 1 g
MgSO4.7H2O 0.5 g
Dichloran (0.2% in ethanol) 1.0 ml
Glycerol 220 g
Chloramphenicol 0.1 g
Agar 15 g
Distilled water 1000 ml
Add minor ingredients and agar to ca. 800 ml distilled water. Steam to dissolve agar, then make to one litre with distilled water. Add 220 g glycerol and sterilize by autoclaving at 121°C for 15 min. The final aw is 0.955: final pH 5.6 ± 0.2. Commercially available (Oxoid).
MEA: Malt extract agar (Oxoid)
Malt extract 30 g
Mycological peptone 5 g
Agar 15 g
Distilled water 1000 ml
Final pH 5.4 ± 0.2
Sterilize by autoclaving at 115°C for 10 min.
Dear Prof……
It is my honour to write to you again. Hope you are getting on well. I had written to you about the identification of a strain in Botryosphaeriaceae on July 5th, 2012.
My name is Feng-hua Tian, a student of Jilin Agricultural University from China. I have started my masters work on Sept 1th, 2010 and Soil Microbial Ecology was selected as my project work. Recently, I was selected as an exchange student for the \"Acetylcholineesterase inhibitors comprising the extracts of Cladonia macilenta purple or culture broth and/or biruloquinone\" in Korean Lichen Research Institute at the Sunchon National University, South Korea. I have been worked there for 6 months and have gained good knowledge on identification of Endophytic fungi.
Since I have knowledge on fungi identification, I would like to continue my work for a Ph.D. degree after completing my masters. So that, I would like to apply for the above position and would like to work under your supervision in your lab.
Sir, I have read many papers published by you and they are very interested. If there is any possibility to do a Ph.D., could you consider me as one of the candidate? I am looking forward to hear your reply.
Thank You.
Yours sincerely,
Feng-hua Tian
