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专利名称：Single primer PCR amplification of RNA发明人：Lyle Arnold,Erik Bjeldanes,Steve Daniel申请号：US09617578申请日：20000717公开号：US06379932B1公开日：20020430
摘要：The invention provides methods and compositions for amplifying RNAsequences by (a) hybridizing to a target RNA a first primer comprising a 3′ target RNAhybridizing sequence and a first 5′ defined amplifyable sequence; (b) extending the firstprimer with a reverse transcriptase to form a first cDNA strand; (c) hybridizing to the firstcDNA strand a second primer comprising a 3′ random cDNA hybridizing sequence and asecond 5′ defined amplifyable sequence; (d) extending the second primer with a DNApolymerase to form a second cDNA strand; and (e) amplifying the second cDNA strandwith a third primer comprising the first 5′ defined amplifyable sequence.
申请人：INCYTE GENOMICS, INC.
代理人：Richard Aron Osman
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