DAPI
产品编号
产品名称
包装
C1002 DAPI (5mg/ml) 0.2ml 产品简介:
DAPI,即2-(4-Amidinophenyl)-6-indolecarbamidine dihydrochloride,也称DAPI dihydrochloride,分子式为C16H15N5 · 2HCl,分子量为350.25,CAS Number 28718-90-3。
DAPI是一种可以穿透细胞膜的蓝色荧光染料。和双链DNA结合后可以产生比DAPI自身强20多倍的荧光。和EB(ethidium bromide)相比,对双链DNA的染色灵敏度要高很多倍。
DAPI染色常用于细胞凋亡检测,染色后用荧光显微镜观察或流式细胞仪检测。DAPI也常用于普通的细胞核染色以及某些特定情况下的双链DNA染色。
DAPI的最大激发波长为340nm,最大发射波长为488nm;DAPI和双链DNA结合后,最大激发波长为3nm,最大发射波长为454nm。
本DAPI溶液用水配制。
用于细胞核染色时,推荐的DAPI工作浓度为0.5-10μg/ml。
包装清单:
产品编号 产品名称 包装 C1002 DAPI (5mg/ml) 0.2ml — 说明书 1份
保存条件:
-20℃避光保存,一年有效。
注意事项:
DAPI对人体有一定刺激性,请注意适当防护。
荧光染料都存在淬灭的问题,建议染色后尽量当天完成检测。
为减缓荧光淬灭可以使用抗荧光淬灭封片液。抗荧光淬灭封片液(P0126)可以向碧云天订购。 为了您的安全和健康,请穿实验服并戴一次性手套操作。
使用本产品的文献:
1. Zhou H, Qian J, Wang J, Yao W, Liu C, Chen J, Cao X.
Enhanced bioactivity of bone morphogenetic protein-2 with low dose of 2-N, 6-O-sulfated chitosan in vitro and in vivo. Biomaterials. 2009 Mar;30(9):1715-24. Epub 2009 Jan 7. 2. Gong Y, Wu J, Huang Y, Shen S, Han X.
Nonylphenol induces apoptosis in rat testicular Sertoli cells via endoplasmic reticulum stress. Toxicol Lett. 2009 Apr 25;186(2):84-95. Epub 2009 Jan 17.
3. Dong HS, Li L, Song ZH, Tang J, Xu B, Zhai XW, Sun LL, Zhang P, Li ZB, Pan QJ, Shi QH, Shen W.
Premeiotic fetal murine germ cells cultured in vitro form typical oocyte-like cells but do not progress through meiosis. Theriogenology. 2009 Jul 15;72(2):219-31. Epub 2009 Apr 9. 4. Chen K, Zhang Q, Wang J, Liu F, Mi M, Xu H, Chen F, Zeng K.
Taurine protects transformed rat retinal ganglion cells from hypoxia-induced apoptosis by preventing mitochondrial dysfunction. Brain Res. 2009 Jul 7;1279:131-8. Epub 2009 May 7. 5. Xin HY, Cheng AC, Wang MS, Jia RY, Shen CJ, Chang H.
Identification and characterization of a duck enteritis virus US3-like gene. Avian Dis. 2009 Sep;53(3):363-9.
6. Ding W, Ju S, Jiang S, Zhu L, Wang Y, Wang H.
Reduced APRIL expression induces cellular senescence via a HSPG-dependent pathway. Pathol Oncol Res. 2009 Dec;15(4):693-701. Epub 2009 May 26. 7. Gong Y, Wu J, Huang Y, Shen S, Han X.
Nonylphenol induces apoptosis in rat testicular Sertoli cells via endoplasmic reticulum stress. Toxicol Lett. 2009;186(2):84-95. Epub 2009 Jan 17.
8. Xie W, Cheng A, Wang M, Chang H, Zhu D, Luo Q.
Molecular cloning and characterization of the UL31 gene from duck enteritis virus. Mol Biol Rep. 2010 Mar;37(3):1495-503. Epub 2009 May 23.
9. Xu H, Wang P, Fu Y, Zheng Y, Tang Q, Si L, You J, Zhang Z, Zhu Y, Zhou L, Wei Z, Lin B, Hu L, Kong X.
Length of the ORF, position of the first AUG and the Kozak motif are important factors in potential dual-coding transcripts. Cell Res. 2010;20(4):445-57. Epub 2010 Feb 16.
10. Zhang Y, Zhou L, Bao YL, Wu Y, Yu CL, Huang YX, Sun Y, Zheng LH, Li YX.
Butyrate induces cell apoptosis through activation of JNK MAP kinase pathway in human colon cancer RKO cells. Chem Biol Interact. 2010;185(3):174-81. Epub 2010 Mar 25.