pQE-30 UA Vector
Positions of elements in bases Vector size (bp)
Start of numbering at XhoI (CTCGAG) T5 promoter/lac operator element T5 transcription start
6xHis tag coding sequence Multiple cloning site I
Cloning site for PCR product Multiple cloning site II
Lambda t0 transcriptional termination region rrnB T1 transcriptional termination region ColE1 origin of replication β-lactamase coding sequence
3504 1-6 7-87 61 127-144 145-173 174-175 176-235 251-345 1107-1205
1681 3299-2439
